Twinkle, Twinkle, Little Star is a song.
- Howie sings it to the viewers, then is joined by every single YTV character.
Mr. Men version
- Mr. Bump sings this song with Mr. Grumpy, Mr. Nervous, Mr. Tall, Little Miss Neat, Mr. Noisy, Mr. Happy, Mr. Clever, Mr. Funny, Little Miss Bossy, Mr. Tickle, Mr. Spendy, Spotted Honking Turtle and Roundy at a sing-along.
- Neville the New Black Engine sings this.